Human VEGF165(Vascular Endothelial Growth Factor165) ELISA Kit

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VEGF165(Vascular Endothelial Growth Factor165) Basic information

Vascular endothelial growth factor (VEGF), originally known as vascular permeability factor (VPF),is a signal protein produced by many cells that stimulates the formation of blood vessels. To be specific, VEGF is a sub-family of growth factors, the platelet-derived growth factor family of cystine-knot growth factors. They are important signaling proteins involved in both vasculogenesis (the de novo formation of the embryonic circulatory system) and angiogenesis (the growth of blood vessels from pre-existing vasculature).This assay has high sensitivity and excellent specificity for detection of Vascular Endothelial Growth Factor 165 (VEGF165).

VEGF165(Vascular Endothelial Growth Factor165) ELISA Kit test method

Feiyue’s Human VEGF165(Vascular Endothelial Growth Factor165) Elisa kit is an ELISA reagent for detection of Neutrophil elastase in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

This kit uses sandwich ELISA to detect the concentration of VEGF165(Vascular Endothelial Growth Factor165), VEGF165(Vascular Endothelial Growth Factor165) -specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standard samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by an additional anti-  VEGF165(Vascular Endothelial Growth Factor165) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of   VEGF165(Vascular Endothelial Growth Factor165) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.