Bovine IL-6(Interleukin 6) ELISA Kit
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Product Descriptions
Bovine IL-6(Interleukin 6) ELISA Kit
Product Name | Bovine IL-6(Interleukin 6) ELISA Kit |
Catalog NO. | FY-EB3988 |
Alias | IL6 ELISA Kit, BSF2 ELISA Kit, HGF ELISA Kit, HSF ELISA Kit, IFNB2 ELISA Kit, CDF ELISA Kit |
Application | IL-6 ELISA Kit allows for the in vitro quantitative determination of IL-6 concentrations in serum, plasma, tissue homogenates and other biological fluids. |
Size | 48T, 96T |
Storage | 2-8 ℃ for 6 months |
Sensitivity | <18.75pg/ml |
Species | Bovine |
Uniprot | P26892 |
CV （%） | Intra-Assay: CV<8% |
Note | For Research Use Only |
Feiyue's Bovine IL-6(Interleukin 6) ELISA Kit is an ELISA reagent for detection of IL-6, plasma or cell with sensitivity, specificity and consistency.
Bovine IL-6(Interleukin 6) Introduciton
IL-6 is secreted by macrophages in response to specific microbial molecules, referred to as pathogen-associated molecular patterns (PAMPs). These PAMPs bind to an important group of detection molecules of the innate immune system, called pattern recognition receptors (PRRs), including Toll-like receptors (TLRs). These are present on the cell surface and intracellular compartments and induce intracellular signaling cascades that give rise to inflammatory cytokine production. IL-6 is an important mediator of fever and of the acute phase response.
Bovine IL-6(Interleukin 6) ELISA Kit Test method
This kit uses sandwich ELISA to detect the concentration of Bovine IL-6.
Bovine IL-6-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Bovine IL-6 antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Bovine IL-6 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.
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