Human GLP1(Glucagon-like peptide 1) ELISA Kit

Reactivity:Human

Assay range:12.35–1,000pg/mL

Sensitivity:< 4.92pg/mL

Detection Method:Competitive Inhibition

Storage:2–8 ℃ for 6 months

CV (%):Intra-Assay: CV<8%; Inter-Assay: CV<10%

Note:For Research Use Only

GLP1(Glucagon-like peptide 1) Basic information

GLP-1 is a 30- or 31-amino-acid-long peptide hormone deriving from the tissue-specific posttranslational processing of the proglucagon peptide. It is produced and secreted by intestinal enteroendocrine L-cells and certain neurons within the nucleus of the solitary tract in the brainstem upon food consumption. The initial product GLP-1 (1–37) is susceptible to amidation and proteolytic cleavage, which gives rise to the two truncated and equipotent biologically active forms, GLP-1 (7–36) amide and GLP-1 (7–37). Active GLP-1 protein secondary structure includes two α-helices from amino acid position 13–20 and 24–35 separated by a linker region.

Human GLP1(Glucagon-like peptide 1) ELISA Kit ELISA Kit ELISA Kit test method

This kit was based on Competitive-ELISA detection method. The microtiter plate provided in this kit has been pre-coated with target. During the reaction, target in the sample or standard competes with a fixed amount of target on the solid phase supporter for sites on the Biotinylated Detection Antibody specific to target. Excess conjugate and unbound sample or standard are washed from the plate, and HRP-Streptavidin (SABC) is added to each microplate well and incubated. Then TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm. The concentration of target in the samples is then determined by comparing the OD of the samples to the standard curve.