Human GPT(Alanine aminotransferase 1) ELISA Kit

Synonyms: GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic — alanine transaminase 1

Catalog NO.:FY-EH4648

Reactivity:Human

Assay range:3.125–200 mU/mL

Sensitivity:<1.875mU/mL

Detection Method:Sandwich ELISA, Double Antibody

Size:48T, 96T

Storage:2–8 ℃ for 6 months

CV (%):Intra-Assay: CV<8%; Inter-Assay: CV<10%

Note:For Research Use Only

Gpt(Alanine aminotransferase 1) Basic information

Alanine transaminase (ALT) is a transaminase enzyme (EC 2.6.1.2). It is also called alanine aminotransferase (ALT or ALAT) and was formerly called serum glutamate-pyruvate transaminase or serum glutamic-pyruvic transaminase (SGPT) and was first characterized in the mid-1950s by Arthur Karmen and colleagues. ALT is found in plasma and in various body tissues but is most common in the liver. It catalyzes the two parts of the alanine cycle. Serum ALT level, serum AST (aspartate transaminase) level, and their ratio (AST/ALT ratio) are commonly measured clinically as biomarkers for liver health. The tests are part of blood panels.

Human Gpt(Alanine aminotransferase 1) ELISA Kit test method

Feiyue’s Human Gpt(Alanine aminotransferase 1) is an ELISA reagent for detection of Gpt(Alanine aminotransferase 1) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

This kit uses sandwich ELISA to detect the concentration of Human Gpt(Alanine aminotransferase 1). Human Gpt(Alanine aminotransferase 1)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Human Gpt(Alanine aminotransferase 1) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Human Gpt(Alanine aminotransferase 1) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. Karmen A, Wroblewski F, Ladue JS (January 1955). “Transaminase activity in human blood”. The Journal of Clinical Investigation. 34 (1): 126–31. doi:10.1172/JCI103055. PMC 438594. PMID 13221663.

2. Ghouri N, Preiss D, Sattar N (September 2010). “Liver enzymes, nonalcoholic fatty liver disease, and incident cardiovascular disease: a narrative review and clinical perspective of prospective data”. Hepatology. 52 (3): 1156–61. doi:10.1002/hep.23789. PMID 20658466. S2CID 5141849.