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Human IL-4(Interleukin 4) ELISA Kit

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Human IL-4(Interleukin 4) ELISA Kit Basic Information 

Product Name

Human IL-4(Interleukin 4) ELISA Kit

Catalog NO.

FY-EH3984

Alias

IL-4 ELISA Kit, Interleukin 4 ELISA Kit, IL4 ELISA Kit, BCGF-1 ELISA Kit, BCGF1 ELISA Kit, BSF-1 ELISA Kit, BSF1 ELISA Kit

Size

48T, 96T

Storage

2-8 ℃ for 6 months

Sensitivity

< 18.75pg/ml

Species

Human

Uniprot

P05112

CV (%)

Intra-Assay: CV<8%
Inter-Assay: CV<10%

Note

For Research Use Only

Feiyue’s Human IL-4 is an ELISA reagent for detection of IL-4 in human serum, plasma or cell with sensitivity, specificity and consistency.

Human IL-4(Interleukin 4) Introduciton

IL4 is involved in at least several B cell activation processes and other cell types. It is a costimulator of DNA synthesis. It induces the expression of MHC class II molecules on resting B cells. It enhances the secretion and cell surface expression of IgE and IgG1. It also regulates the low-affinity Fc receptor expression of IgE (CD23) on lymphocytes and monocytes. Genetic variation of IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction.

Human IL-4(Interleukin 4) ELISA Kit Test method

This kit uses sandwich ELISA to detect the concentration of human IL-4.

Human IL-4-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate.Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-human IL-4 antibody with HRP-Streptavidin.TMB solution is added to react with the sandwich forming optical signal measured by microplate reader.The concentration of human IL-4 in the sample can be calculated by comparing the absorbance of the sample with the standard curve.  

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