Rabbit IL-1β(Interleukin 1 Beta) ELISA Kit

Synonyms: IL1-B; IL1-Beta; IL1F2; IL-1β; Interleukin-1 Family Member 2; Catabolin

Reactivity：Rabbit

Assay range：7.8–500pg/mL

Sensitivity：< 2.8pg/mL

Detection Method：Double-antibody Sandwich

Size：48T, 96T

Storage：2–8 ℃ for 6 months

CV (%)：Intra-Assay: CV<8%；Inter-Assay: CV<10%

Note：For Research Use Only

IL-1β(Interleukin 1 Beta) Basic information

The fever-producing property of human leukocytic pyrogen (interleukin 1) was purified by Dinarello in 1977 with a specific activity of 10–20 nanograms/kg. In 1979, Dinarello reported that purified human leukocytic pyrogen was the same molecule that was described by Igal Gery in 1972. He named it lymphocyte-activating factor (LAF) because it was a lymphocyte mitogen. It was not until 1984 that interleukin 1 was discovered to consist of two distinct proteins, now called interleukin-1 alpha and interleukin-1 beta. IL-1β is a member of the interleukin 1 family of cytokines. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.

Rabbit IL-1β(Interleukin 1 Beta) ELISA Kit test method

Feiyue’s Rabbit IL-1β(Interleukin 1 Beta) is an ELISA reagent for detection of IL-1β(Interleukin 1 Beta) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

This kit uses sandwich ELISA to detect the concentration of Rabbit IL-1β(Interleukin 1 Beta). Rabbit IL-1β(Interleukin 1 Beta)-specific monoclonal antibody has been pre-coated in the wells of the supplied microplate. Standards samples and controls are added to interact with the immobilized antibody. A sandwich complex is formed by additional anti-Rabbit IL-1β(Interleukin 1 Beta) antibody with HRP-Streptavidin. TMB solution is added to react with the sandwich for ming optical signal measured by microplate reader. The concentration of Rabbit IL-1β(Interleukin 1 Beta) in the sample can be calculated by comparing the absorbance of the sample with the standard curve.

Reference:

1. GRCh38: Ensembl release 89: ENSG00000125538 — Ensembl, May 2017

2. GRCm38: Ensembl release 89: ENSMUSG00000027398 — Ensembl, May 2017

3. “Human PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.

4. “Mouse PubMed Reference:”. National Center for Biotechnology Information, U.S. National Library of Medicine.